Generation of stable brain cell cultures from embryonic zebrafish to interrogate phenotypes in zebrafish mutants of neurodevelopmental disorders
Odierna G, Stednitz S, Pruitt A, Arnold J, Hoffman E, Scott E. Generation of stable brain cell cultures from embryonic zebrafish to interrogate phenotypes in zebrafish mutants of neurodevelopmental disorders. Journal Of Neuroscience Methods 2025, 418: 110426. PMID: 40086601, DOI: 10.1016/j.jneumeth.2025.110426.Peer-Reviewed Original ResearchBrain cell culturesCell culturesCell typesNeuronal culture protocolCultured primary neuronsEmbryonic zebrafishPrimary brain cell culturesZebrafish mutantsCellular consequencesStructural hallmarksMature synaptic connectionsPrimary neuronsCell survivalMammalian tissuesTranscriptional signatureCell linesEmbryonic tissuesZebrafish neuronsZebrafishNeuron purityNeuronal culturesNetwork of neuronsMixed cell typeDays in vitroModel system
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