Coupling clearing and hybridization chain reaction approaches to investigate gene expression in organs inside intact insect heads
Cayrol B, Colella S, Uzest M. Coupling clearing and hybridization chain reaction approaches to investigate gene expression in organs inside intact insect heads. Microscopy Research And Technique 2024, 87: 1926-1932. PMID: 38558482, DOI: 10.1002/jemt.24561.Peer-Reviewed Original ResearchConceptsSecretory cellsSpatial distribution of transcriptsSecretory cells of salivary glandsDetected RNA moleculesDetection of transcriptsDistribution of transcriptsInvestigate gene expressionPigmented cuticleHigh-resolution analysisRNA moleculesCells of salivary glandsHybridization chain reactionAphid headsGene expressionPrincipal glandChain reactionTranscriptionAmplification approachInsect headAcyrthosiphon pisumHybridisation chain reactionCuticleMultiplex approachSmall organismsSHP
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